Toxoplasma gondii IgG (Toxo IgG) ELISA kit description:

The Diagnostic Automation Elisa, Toxoplasma IgG is intended for use in evaluating a patient's
serologic status to toxoplasma gondii infection.

Toxoplasmosis is caused by the intracellular parasite Toxoplasma gondii and may be contracted by
consuming contaminated meat or by contact with cat feces containing oocysts. In adolescence and
adulthood, most infections are subclinical. However, if a pregnant woman contracts toxoplasmosis,
it may be passed through the placenta to the fetus, resulting in congenital toxoplasmosis, which is a
cause of mortality and malformation. Asymptomatic infants may develop anomalies later in life. The DIAGNOSTIC AUTOMATION ELISA Toxoplasma IgG is an accurate serologic method to detect Toxoplasma antibody for clinical identification of toxoplasmosis.

Purified Toxoplasma gondii antigen is coated on the surface of microwells. Diluted patient serum is
added to wells, and the Toxoplasma gondii IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibodyantigen
complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of thecolor generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.