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Verotoxin ELISA Kit

Name

Verotoxin ELISA Test

Full name

Food Verotoxin Antigen ELISA Test Kit

Category Name Food ELISA kits
Test 96

Item #:                    8328-3   Quantity:               

Verotoxin ELISA Kit


Verotoxin ELISA Kit

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Verotoxin ELISA Kit description:




Diagnostic Automation Inc. offers an E. coli Verotoxin Food ELISA Kit that is an Enzyme-linked immunosorbent assay to screen food products for the presence of Verotoxin. This Verotoxin Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect verotoxin antigen in food.



Materials Provided with Verotoxin ELISA Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated rabbit anti-Verotoxin
(VT1 &VT2) antibodies
2. Test strip holder
3. Negative Control: One (1) vial containing 2 ml of a buffered base.
4. Positive Control: One (1) vial containing 2 ml of inactivated Verotoxin antigen
5. Reagent 1: monoclonal anti-Verotoxin antibodies
6. Reagent 2: anti-mouse antibody conjugated to horseradish peroxidase
7. Chromogen: chromogen tetramethylbenzidine (TMB)
8. Wash concentrate solution 20x
9. Stop solution: 1 M phosphoric acid

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




Verotoxin ELISA Test Kit Background Information:
The Verotoxin (VT), or shiga-like toxin family is a group of closely related toxins produced by certain strains of Escherichia coli. These strains are a significant cause of human hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). In addition, they are both water borne and food borne and may also be transmitted from person-to-person by the oral-fecal route. In adults, illness caused by Verotoxin may last several days, while in children and the elderly, the illness can be fatal. Infections occur as sporadic illnesses or outbreaks, and are distributed throughout North and South America, Europe, and Asia. The peak incidence of infection occurs during summer months. In the past, lengthy and cumbersome diagnostic procedures have been utilized for the detection of verotoxin. Currently, simple to perform enzyme-linked immunosorbent assays (ELISA), like this Verotoxin Food ELISA Test Kit, are being used as an alternative to these longer tedious methods. These antigen-detection assays provide a cost effective tool for the management of diarrheal disease with an accurate and rapid test.




Verotoxin Elisa Test Principle:
The Verotoxin Food ELISA Kit is a quantitative test based on the principle of the enzyme-linked immunosorbent assay. During the first incubation, Verotoxin antigen in the sample is captured by antibodies attached to the wells of the microtiter plate provided in Verotoxin ELISA Kit.




Diagnostic Automation Inc offers several popular Food Allergen ELISA Kits: Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Peanut ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.




Product Note:

When assaying Diagnostic Automation, Inc.  Verotoxin ELISA test kit some additional materials are reuired including  Stomacher or blender,  Shaker,  incubator,  Microplate ELISA reader capable of reading bichromatically at 450-650 nm.  Pipetter  100 ul, Disposable micropipette tips , Microbiological media and antibiotics for preparation of necessary enrichment broths and plating media, appropriate containers for storage and disposal of materials potentially contaminated with infectious agents,  Data record sheets and Disinfecting Solution. Store test kit at 2-8 C.