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Walnut ELISA kit


Walnut ELISA Test

Full name

Food Walnut ELISA Test Kit

Category Name Food ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Peroxidase conjugated
Detection Range Qualitative elisa assay- Positive, Negative Controls

Item #:                    5145-8   Quantity:               

Walnut ELISA kit

Walnut ELISA kit

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Walnut ELISA kit description:

Diagnostic Automation Inc. offers a Walnut ELISA Kit that is an Enzyme Immunosorbent Assay for the Qualitative determination of Walnut in food. This Walnut ELISA Kit is a rapid Food Allergen ELISA test which is designed to provide a sensitive and convenient method for screening food samples for walnut residues.

Materials Provided with Walnut ELISA Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with anti-walnut antibodies
2. Walnut Standards (0; 2; 6; 20; 60 ppm of walnut): 5 vials with 1.0 mL each
3. Conjugate (anti-walnut-peroxidase): 15 mL
4. Substrate Solution (TMB): 15 mL
5. Stop Solution (0.5 M H2SO4): 15 mL
6. Extraction and sample dilution buffer (Tris): 2 x
7. Washing Solution (PBS + Tween 20): 60 mL as 10x
8. Plastic bag to store unused microtiter strips.
9. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength

Walnut ELISA Test Kit Background Information:
Walnut (Juglans regia) belongs to the walnut plants (Juglandaceae). With 15% the fraction of proteins in walnuts is high. Some of these proteins are known for being allergenic, such as rJug r 1 and rJug r 4. Many of them are heat resistant making them stable to different production processes. For this reason walnut represents an important food allergen. For walnut allergic persons, hidden walnut allergens in food are a critical problem. Already very low amounts of walnut can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, walnut allergic persons must strictly avoid the consumption of walnuts or walnut containing food. Cross-contamination, mostly in consequence of the production process, is often noticed. The chocolate production process is a representative example. This explains why in many cases the presence of walnut residues in food cannot be excluded. For this reason sensitive detection systems such as this Walnut allergy test kit for walnut residues in foodstuffs are required.

Walnut ELISA Kit Test Principle:
The Walnut ELISA Kit is a quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against walnut proteins is bound on the surface of a microtiter plate provided in this Walnut ELISA Kit.

Diagnostic Automation Inc offers several popular Food Allergen ELISA Kits: Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Peanut ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

When utilizing Diagnostic Automation, Inc. Walnut ELISA test kit, please note that due to high risk of cross-contamination all applied instruments like applicator, mortar, glass vials etc. have to be cleaned thoroughly before and after each sample. Walnut proteins adhere very strongly to different surfaces. To identify possible cross-contamination caused by previous extractions it is strongly recommended to note the sequence of the extractions. The limit of detection (LOD) of the DAI Walnut test is 0.35 ppm. The limit of quantification (LOQ) of the DAI Walnut test is 2 ppm.  Due to the variety of sample matrices and their influence on the blank, results less than the LOQ should be treated as negative.