The Diagnostic Automation Free T4 ELISA is intended for the quantitative determination of the Free Thyroxine (T4) concentration in human serum.





Material Provided with Free T4 ELISA Kit:
1. Human Serum References: Six human serum based reference calibrators
2. fT4- Enzyme Reagent: thyroxine-HRP conjugate
3. Microplate: Antibody Coated Wells
4. Wash Solution Concentrate
5. Substrate A: TMB in acetate buffer
6. Substrate B: H2O2 in acetate buffer
7. Stop Solution: 1N HCl
8. Product Instructions

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm





Free T4 ELISA Kit Background Information:
Thyroxine, the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. The main carrier is thyroxine-binding globulin (TBG). However, only the free thyroxine is responsible for the biological action. The increase in total thyroxine associated with pregnancy, oral contraceptives and estrogen therapy occasionally result in total T4 levels over the limits of normal while the free thyroxine concentration remains in the normal reference range. Masking of abnormal thyroid function can also occur in both hyper and hypothyroid conditions by alterations in the TBG concentration. The total T4 can be elevated or lowered by TBG changes such that the normal reference levels result. The free thyroxine concentration can help in uncovering the patient's actual clinical status.





Free T4 ELISA Test Principle:
The Free T4 ELISA test is based on the principle of a solid phase enzyme-linked competitive enzyme immunoassay. The essential reagents required for a solid phase enzyme immunoassay include immobilized antibody, enzyme-antigen conjugate and native antigen. Upon mixing immobilized antibody, enzyme-antigen conjugate and a serum containing the native free antigen, a competition reaction results between the native free antigen and the enzyme-antigen conjugate for a limited number of insolubilized binding sites. For additional details please refer to the instructions for use.







Diagnostic Automation Inc. also provides other Thyroid ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .