Diagnostic Automation The ELISA Epstein-Barr Virus Viral Capsid Antigen (EBV-VCA) IgG Test System is an enzyme-linked immunosorbent assay (ELISA) designed for the qualitative detection of IgG class antibodies to Epstein-Barr Virus Viral Capsid Antigen in human serum.




Materials Provided with EBV VCA IgG ELISA kit:
1. Microwell strips: inactivated EBV-VCA antigen coated wells
2. Sample Diluent
3. Calibrator
4. Negative Control
5. Positive Control
6. Conjugate: Conjugated HRP-goat anti-human IgG
7. TMB Enzyme Conjugate
8. Wash Buffer Concentrate (10X)
9. Stop Solution

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength



EBV-VCA IgG ELISA Test Background Information:
EBV is classified as a member of the herpes-virus family based upon its characteristic morphology. EBV infection may demonstrate a wide spectrum of clinical symptoms. The majority of primary EBV infections is transmitted via saliva, occurs during childhood, and is subclinical. Antibody titers to specific EBV antigens correlate with different stages of IM. Both IgM and IgG antibodies to the viral capsid antigen (VCA) peak 3 to 4 weeks after primary EBV infection. IgM anti-VCA declines rapidly and is usually undetectable after 12 weeks. IgG anti-VCA titers decline slowly after peaking but last indefinitely. Antibodies to EBV nuclear antigen (EBNA) detected by anti-complement immunofluorescence develop from 1 month to 6 months after infection; and, like anti-VCA, persist indefinitely. Antibodies to EBNA indicate that the EBV infection was not recent. Antibodies to EA may appear transiently for up to three months or longer during the acute phase of IM in 85% of patients. Elevated levels of anti-EA and IgG anti-VCA may be detected in patients with chronic or recurrent illness suspected of being caused by EBV. However, a diagnosis of chronic EBV should not be based on the presence of antibodies to EA since elevated anti-EA titers may also be found in patients with other diseases as well as in healthy individuals with past EBV infections.



EBV-VCA IgG ELISA Test Principle:
Purified EBV-VCA antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the EBV-VCA IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time.



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