Beta 2 Microglobulin ELISA kit
| Name |
Beta 2 Microglobulin ELISA Test |
|---|---|
| Full name |
Human Beta 2 Microglobulin ELISA Test Kit |
| Category Name | Cancer ELISA kits |
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | ELISA principle- Peroxidase Conjugated Competitive ELISA |
| Detection Range | 0-20 ug/ml |
| Sample | 10ul serum |
| Specificity | 96% |
| Sensitivity | 2.0 ug/mL |
| Total Time | ~80min |
| Shelf Life | 12 Months from the manufacturing date |
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Beta 2 Microglobulin ELISA kit description:
The Beta-2 Microglobulin ELISA test is an enzyme immunoassay (EIA) for the measurement of Beta-2 Microglobulin in serum as an aid in the diagnosis of active rheumatoid arthritis and kidney disease.
Material Provided with B2 Microglobulin ELISA Kit:
1. Microplate: wells coated with Anti-Beta-2 MG antibody
2. Sample diluent
3. Enzyme conjugate reagent
4. B2MG reference standards
5. TMB Substrate
6. Stop Solution
7. Wash Buffer Concentrate (50X)
Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm
Beta-2 Microglobulin ELISA Kit Background Information:
Human Beta-2 Microglobulin (B2MG) is an 11.8 kD protein identical to the light chain of the HLA-A, -B, and -C antigen. B2MG is expressed on nucleated cells, and is found at low levels in the serum and urine of normal individuals. B2MG concentrations are increased in inflammatory diseases, some viral diseases, renal dysfunction, and autoimmune diseases. Number of studies are available which explain the interpretation of B2MG serum levels in assessing the status of individuals with various clinical conditions.
Beta-2 Microglobulin ELISA Test Principle:
The Beta-2 Microglobulin ELISA assay allows the quantitative determination of B2MG from serum. In this assay, the B2MG in the samples is bound to an available excess of monoclonal antibodies against B2MG, which are immobilized to the surface of the microtiter wells. After a washing step to remove all foreign substances, the quantification of bound B2MG is carried out by adding an enzyme horseradish peroxidase or HRPO labeled antibody, which also binds to the B2MG. The amount of bound enzyme is directly proportional to the B2MG content. For additional details please refer to the instructions for use.
Notable features of Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results
Product inserts for all Cancer ELISA kits follow a similar method. See the Beta-2 Microglobulin ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.
Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .