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Rubella IgG CLIA kits - (Chemiluminescence Immuno Assay)

Name

Rubella IgG CLIA kits - (Chemiluminescence Immuno Assay)

Category Name Infectious Disease Assays
Test 96

Item #:                    9069-11   Quantity:               

Rubella IgG CLIA kits - (Chemiluminescence Immuno Assay)


Rubella IgG CLIA kits - (Chemiluminescence Immuno Assay)

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Rubella IgG CLIA kits - (Chemiluminescence Immuno Assay) description:




Rubella IgG Chemiluminescence ELISA is intended for use in evaluating a patient's serologic status to
rubella virus infection. It is also used to evaluate paired sera for the presence of a significant increase in
specific IgG as indicative of a recent or current rubella virus infection.

Rubella is a herpes virus. Generally rubella is considered a mild adolescence disease. However a
maternal infection could be transmitted through the placenta to the fetus, causing congenital rubella.
Congenital rubella may result in chronic cardiac disease, growth retardation, hepatosplenomegaly,
malformations and other severe anomalies. Children born asymptomatic may develop these abnormalities later in life. To reduce risk of such severe complications, accurate serological methods
must be performed to determine the serologic status of childbearing aged women. The presence of
rubella specific IgG in the bloodstream attests immunity to rubella. A woman tested to be non-immune
can be educated on the availability of vaccination. An increase in rubella IgG denotes an acute infection
and differentiates rubella from other exanthematous diseases. Expecting women with current rubella
infection should be counseled on the consequences of congenital infection.

Purified Rubella antigen is coated on the surface of microwells. Diluted patient serum is added to wells,
and the Rubella IgG specific antibody, if present, binds to the antigen. All unbound materials are washed
away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and substrate A & substrate B mixture is added. The light generated (RLU) is
proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell
luminometer compared in a parallel manner with calibrator and controls.

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