Cortisol ELISA kit description:

The Diagnostic Automation Cortisol ELISA kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Cortisol concentration in human serum and plasma.




Material Provided with Cortisol ELISA Kit:
1. Cortisol EIA Calibrators: serum reference at concentrations of 0, 1, 4, 10, 20 &50 ug/dl
2. Cortisol ELISA Test Enzyme Reagent of Cortisol Analog-HRP
3. Cortisol ELISA Steroid Conjugate Buffer
4. Cortisol EIA kit Biotin Reagent of anti-cortisol biotinylated
5. Microplate coated with 1.0 ug/ml streptavidin
6. Cortisol ELISA Test Wash Concentrate Solution
7. Cortisol EIA kit Substrate A: TMB
8. Cortisol EIA kit Substrate B: H2O2
9. Stop Solution
10. Product Insert

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




Cortisol ELISA Kit Background Information:
17-Hydroxyprogesterone (17-OH progesterone or 17OHP) is a C-21 steroid hormone produced in the adrenal gland and gonads, during thesynthesis of glucocorticoids and sex steroids. 17OH progesterone is a precursor of cortisol that accumulates in the case of adrenal 21-hydroxylase deficiency and decreased after replacement therapy with cortisol. High values are found in congenital adrenal hyperplasia. Serum 17 a-OHP levels are age-dependent, with peak levels observed during fetal life and the immediate postnatal period. Levels for both sexes remain at constant low levels during childhood, and then progressively increase during puberty and adulthood. As with cortisol, serum 17OHP levels normally have an ACTH-dependent diurnal variation, with peak levels in the morning and a nadir at night. In addition, ovarian production of 17OHP increases during the luteal phase of the menstrual cycle. 17a OHP is a natural progestin and in pregnancy increases in the third trimester primarily due to fetal adrenal production. Measurements of levels of 17-hydroxyprogesterone are useful in the evaluation of patients with suspected congenital adrenal hyperplasia as the typical enzymes that are defective, namely 21-hydroxylase and 11b-hydroxilase, lead to a build-up of 17OHP. In contrast, the rare patient with 17a-hydroxylase deficiency will have very low or undetectable levels of 17OHP. Elevated serum 17 a-OHP levels at baseline and/or after ACTH stimulation have also been reported in other forms of adrenal hyperplasia.





Cortisol ELISA Test Principle:
Cortisol ELISA Test Kit is based on Competitive Enzyme Immunoassay. The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen. Upon mixing biotinylated antibody, enzyme-antigen conjugate and a serum containing the native antigen a competition reaction results between the native antigen and the enzyme-antigen conjugate for a limited number of antibody binding sites. A simultaneous reaction between the biotin attached to the antibody and the streptavidin immobilized on the microwells occurs. This effects the separation of the antibody bound fraction after decantation or aspiration. The enzyme activity in the antibody-bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained. For additional details please refer to the instructions for use.




Advantages of Cortisol EIA Kit:

1. The Diagnostic Automation Inc. Cortisol EIA Kit uses a specific monoclonal
anticortisol antibody, and does not require prior sample extraction of serum or plasma.

2. Cross-reactivity to other naturally-occurring steroids is low.

3. The employment of several serum references of known cortisol concentration
permits construction of a graph of activity and concentration. From comparison to
the dose response curve, an unknown specimen's activity can be correlated with
cortisol concentration with higher precision.






Diagnostic Automation Inc. also provides other Steroid ELISA Kits:

http://www.rapidtest.com/index.php?product=Steroid-ELISA-kits&cat=14


For more information about these Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative
at 818-591-3030.
*Not to be sold, shipped, or distributed in United States.