The Diagnostic Automation, Inc. ANCA Screen ELISA test kit is for the qualitative detection of anti-Myeloperoxidase and/or anti-Proteinase-3 IgG antibody in human serum. The ANCA ELISA test kit is intended to be used as an aid in the diagnosis of various autoimmune vasculitic disorders characterized by elevated levels of antineutrophil cytoplasmic antibodies (ANCA).




Material Provided with ANCA Screen IgG ELISA Kit:
1. ANCA Screen IgG EIA Kit Plate: 96 wells strips coated with a mixture of MPO & PR3 antigen
2. Conjugate: Conjugated HRP-goat anti-human IgG (y chain specific)
3. ANCA Screen IgG Test Positive Control (Human Serum)
4. Calibrator (Human Serum)
5. Negative Control (Human Serum)
6. ANCA Screen IgG EIA Test Sample Diluent
7. TMB
8. ANCA Screen IgG ELISA Kit Stop Solution
9. Wash Buffer Concentrate (10X)

Materials & Instrumentations required but not included:

1. Multichannel pipette & tips
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader at 450nm




ANCA Screen ELISA test Background Information:
Anti-neutrophil cytoplasmic antibody (ANCA) was initially described by Davies, et al in 1982. Since this initial discovery, ANCA has been found to be associated with a number of Systemic Vasculitides (SV). ANCA is now recognized to include two primary specificities: C-ANCA directed against Proteinase-3 (PR-3), and P-ANCA directed against Myeloperoxidase (MPO). Testing for both P-ANCA and C-ANCA is highly recommended in the laboratory workup of patients who present with clinical features suggestive of SV. The clinical syndromes most frequently associated with ANCA are as follows:
Wegener s granulomatosis
Polyarteritis
"Overlap" Vasculitis
Idiopathic Crescentic Glomerulonephritis (ICGN)
Kawasaki Disease



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ANCA Screen ELISA test kit Principle:
The Diagnostic Automation Inc. ANCA Screen ELISA test system is designed to detect IgG class antibodies to MPO and/or PR3 in human sera. Wells of plastic microwell strips are sensitized by passive absorption with a mixture of MPO and PR3 antigen.




Notable features of the Autoimmune Disease ELISA Kits:
- User-friendly directions and explanation of test procedures
- Simple and safe reagent preparation
- Clear instructions on specimen collection
- Comprehensive package of required materials
- Explicit quality control and storage guidelines
- Reliable and easy-to-read test results




Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. Most Autoimmune Disease ELISA kits follow a similar method. See the ANCA Screen IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.