The Anti Tissue Transglutaminase IgA ELISA Test is an Indirect-solid phase enzyme immunometric assay for the quantitative detection of IgA antibodies to transglutaminase in serum or plasma. The Anti Tissue Transglutaminase IgA ELISA is an aid in identifying celiac disease and dermatitis herpetiformis.




Materials provided with Anti-Tissue Transglutaminase IgA Elisa kit:

1. Anti-Tissue Transglutaminase IgA Standards, 6 vials
Human serum: STD0, STD1, STD2, STD3, STD4, STD5
2. Controls: human serum, 2 vials
Negative Control
Positive Control
3. Sample Diluent
4. Conjugate: Anti h-IgA conjugated
5. Microplate: wells coated with human recombinant tissue transglutaminase
6. TMB Substrate
7. Stop Solution: Sulphuric acid 0.15M
8. Wash Solution:10X

Materials required, not provided
1. Distilled water.
2. EIA kit Microplate washer
3. EIA kit Microplate Reader with 450nm wavelength




Anti Tissue Transglutaminase IgA ELISA Test Background Information:
Celiac disease is characterized by chronic inflammation of the intestinal mucosa and
flattening of the epithelium or positive (villous atrophy). The origin of the celiac
disease is the intolerance to gluten, the protein of wheat, rye and barley. The main
symptoms are diarrhea, gastrointestinal problems, anemia, fatigue, psychiatric
problems and other diverse side effects. In some cases patients may be asymptomatic. The enzyme tissue Transglutaminase (tTG) has been reported to be the main, if not sole, target for endomysial antibodies. These antibodies fall once a gluten-free diet has begun, thus facilitating monitoring of dietary compli-ance.




Anti Tissue Transglutaminase IgA ELISA Test Principle:
Diagnostic Automation Inc. offers a Transglutaminase IgA ELISA Kit which is an Indirect solid-phase enzyme immunometric assay for the quantitative detection of IgA antibodies to transglutaminase in serum or plasma. The Transglutaminase IgA ELISA kit, like many ELISA KITS, is for in-vitro diagnostic laboratory use only. As in other Autoimmune ELISA Kits, the principle of the Anti-Tissue Transglutamiase IgA ELISA kit is a three-incubation process. The Transglutaminase IgA ELISA Kit is used as an aid in identifying celiac disease and dermatitis herpetiformis.



Notable features of Autoimmune ELISA Kits:
- User-friendly directions and explanation of test procedures
- Simple and safe reagent preparation
- Clear instructions on specimen collection
- Comprehensive package of required materials
- Explicit quality control and storage guidelines
- Reliable and easy-to-read test results

Product inserts for most Autoimmune ELISA kits follow a similar method. See the Transglutaminase IgA ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.


For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030