Vitamin H (Biotin) in food ELISA test kit is an Enzyme Immunoassay for the quantitative determination of Biotin (Vitamin H) in Food.




Materials Provided with Biotin Elisa Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated Avidin
2. Biotin Standards (0; 1; 2.5; 5; 10; 25 ng/mL): 6 vials with 0.5 mL each
3. Conjugate (Biotin-Alkaline Phophatase): 15 mL
4. Substrate Solution (TMB): 15 mL
5. Stop Solution (0.5 M H2SO4): 15 mL
6. Standard/Sample Diluent (PBS): 2 x
7. Washing Solution (PBS + Tween 20): 10x
8. Two plastic foils to cover the strips during the incubation
9. Plastic bag to store unused microtiter strips
10. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader




Biotin ELISA Kit Background Information:
Biotin (Vitamin H) serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysin residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide. The awareness of the population for a good health and its interest in healthy nutrition has increased significantly during the last years. After the content of vitamins in his nourishment has gained importance for the consumer, food has partially been vitaminized by the manufacturer. When there exists a lack of biotin, seborrhoea, dermatitis, anorexia, muscle pain, tiredness and nervous disorders can appear. As biotin is synthesized by the human intestinal flora, deficiency symptoms are rare, appear however after excessive ingestion of raw egg white, which can be explained by its content of biotinbinding avidin.



Biotin ELISA Test Principle:
The Diagnostic Automation Biotin (Vitamin H) quantitative test is based on the principle of the enzyme linked immunosorbent assay. Avidin, which shows a high affinity to biotin, is bound on the surface of a microtiter plate.





Diagnostic Automation Inc. offers several popular Food ELISA Test Kits: Fumonisin ELISA Kit, Streptomycin ELISA Kit, Aflatoxin M1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.