This C-Peptide ELISA test kit is a solid phase enzyme linked immunosorbent assay for measurement of C-peptide concentration in serum.




Materials Provided with C-Peptide ELISA test kit:
A. C-Peptide Calibrators: Six vials of references for C-Peptide antigen at levels of
0(A), 0.2(B), 1.0(C), 2.0(D), 5.0(E), and 10.0(F) ng/ml
B. C-Peptide Enzyme Reagent: containing enzyme labeled biotinylated monoclonal mouse
C. Streptavidin Plate: 96 well microplate coated with sheep anti-triiodothyronine serum
D. Wash Solution Concentrate: containing a surfactant in buffered saline
E. Substrate A: containing tetramethylbenzidine (TMB)
F. Substrate B: containing hydrogen peroxide (H2O2)
G. Stop Solution: containing a strong acid (1.0 N HCI)
H. Product Instructions

Materials required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength
5. Quality Control Materials




C-Peptide ELISA test Background Information:
Diabetes is one of the leading causes of disability and death in the U.S. It affects an estimated 16 million Americans, about one third of them do not even know they have the disease. The most common forms of diabetes are type 1 and type 2. Both insulin and C-Peptide are produced by enzymatic cleavage of proinsulin. Proinsulin is stored in the secretory granuleof pancreatic Beta -cells and is split into a 31 amino acid connecting peptide (C-Peptide; MW 3600) and insulin (MW 6000). C-Peptide is devoid of any biological activity but appears to be necessary to maintain the structural integrity of insulin. Although insulin and C-Peptide are secreted into portal circulation in equimolar concentrations, fasting levels of C-Peptide are 5-10 fold higher than those of insulin owing to the longer half-life of C-Peptide. The liver does not extract C-Peptide however; it is removed from the circulation by degradation in the kidneys with a fraction passing out unchanged in urine. Hence urine CPeptide levels correlate well with fasting C-Peptide levels in serum. The glucagon stimulated C-Peptide determination is often used for differential diagnosis of insulin-dependent from non-insulin-dependent diabetic patients. In-vitro determination of insulin and C-Peptide levels help in the differential diagnosis of liver disease, acromegaly, Cushing's syndrome, familial glucose intolerance, insulinoma, renal failure, ingestion of accidental oral hypoglycemic drugs or insulin induced factitious hypoglycemic.




C-Peptide ELISA Test Principle
The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies (Ab). (enzume conjugated and immobilized), with different and distinct epitope recognition, in excess, and native antigen (Ag). In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal C-peptide antibody.



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