Cryptosporidium ELISA Kit is an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in feces.



Material Provided with Chagas ELISA kit:
1. Test Strips Microwells: 96 test wells anti-Cryptosporidium polyclonal antibodies
2. Enzyme Conjugate: anti-Cryptosporidium antibodies conjugated-HRP
3. Positive Control: diluted Cryptosporidium positive formalinized stool supernatant
4. Negative Control: diluted Cryptosporidium negative formalinized stool supernatant
5. TMB Substrate Solution: TMB and peroxid
6. Wash Concentrate 20X
7. Dilution Buffer: buffered protein solution containing Thimerosal
8. Stop Solution: 5% phosphoric acid

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm and a 620 - 650 nm filter




Cryptosporidium ELISA kit Background Information:
Cryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men. Diagnosis of Cryptosporidium infections was done originally by direct detection techniques. However, this method relies on an experienced technician and subsequent observation of intact organisms. One important alternative has been the development of an antigen capture ELISA for use with stools with comparable sensitivity to experienced microscopic examinations.




Cryptosporidium ELISA Test Principle:
During the first incubation, Cryptosporidium specific antigen present in the stool specimens are captured by antibodies attached to the microwells. The wells are incubated and washed before anti Cryptosporidium antibodies conjugated to horseradish peroxidase are added. The enzyme conjugate will sandwich any antigen bound to the wells. After washings to remove unbound enzyme, a chromogen is added which develops a blue color in the presence of the enzyme complex. The stop solution ends the reaction and turns the blue color to yellow.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Cryptosporidium ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.