The Diagnostic Automation, Inc. offers a Deoxynivalenol quantitative test is based on the
principle of the enzyme-linked immunosorbent assay. Deoxynivalenol ELISA represents a highly sensitive detection system and is particularly capable of the quantification of deoxynivalenol contaminations in cereals, beer and soy.




Materials Provided with Deoxynivalenol Elisa Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with anti-mouse antibody
2. Deoxynivalenol Standards (0, 2, 8, 20, 40, 80 ng/mL): 6 vials with 1 mL each
3. Anti-Deoxynivalenol Antibody (mouse): 6 mL
4. Conjugate (Deoxynivalenol-Peroxidase): 6 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample diluent (PBS) 2x
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Two plastic foils to cover the strips during the incubation
10. Plastic bag to store unused microtiter strips
11. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




Deoxynivalenol ELISA Kit Background Information:
Deoxynivalenol (DON, Vomitoxin) in addition to zearalenone, the fumonisines and other trichothecenes belongs to the fusarium toxins. These toxins are already produced on the field in consequence of a contact of the cereals by fusarium species. Acute toxic dosages result in sickness and emesis. Deoxynivalenol is a gastrointestinal irritant and an inhibitor in protein synthesis. Farm animals react with a delay of growth and a depressed immune system resulting in a higher sensitivity for infections.
Since July 1st, 2006 the following maximum amounts of deoxynivalenol are valid throughout the EU:
Raw cereals 1250-1750 ppb
Flour 750 ppb
Bakery products 500 ppb
Baking ingredients (dry) 750 ppb
Baby food 200 ppb



Deoxynivalenol ELISA Test Principle:
The Diagnostic Automation, Inc. Deoxynivalenol quantitative test is based on the
principle of the enzyme-linked immunosorbent assay. An antibody binding protein is
coated on the surface of a microtiter plate. Deoxynivalenol containing samples or
standards and an antibody directed against deoxynivalenol are given into the wells of
the microtiter plate.




Diagnostic Automation Inc. offers several popular Food ELISA Test Kits: Fumonisin ELISA Kit, Streptomycin ELISA Kit, Aflatoxin M1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.