The E. Histolytica (Amebiasis) ELISA or E. Histolytica IgG ELISA Test is an enzyme linked immunosorbent assay for the qualitative identification of serum IgG antibodies to Entamoeba histolytica.



Material Provided with E. histolytica IgG ELISA kit:
1. Test Strips Microwells: 96 test wells E. histolytica strain NIH-200 antigens
2. Enzyme Conjugate: Protein-A conjugated to peroxidase
3. Positive Control: diluted positive rabbit serum
4. Negative Control: diluted human serum
5. TMB substrate Solution: TMB
6. Wash Concentrate 20X: concentrated buffer and surfactant
7. Dilution Buffer: buffered protein solution
8. Stop Solution: 1 M phosphoric acid

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm and a 620 - 650 nm filter




E. histolytica IgG ELISA kit Background Information:
Generally, the disease of Amebiasis is found in a number of tropical regions where
living conditions and poor sanitation cause significant health problems. Transmission of the disease centers on native populations and tourists traveling from these areas. A protozoan parasite called Entamoeba histolytica is the causing agent of Amebiasis, and the disease usually shows up as intestinal problems. Symptoms are generally mild, but in some cases the organism becomes extraintestinal and can lead to abscesses, primarily affecting the liver. Serological tests are recommended for extra-intestinal diagnosis, making sure to isolate the disease from other diseases of the liver, or ulcerative colitis, for example.




E. histolytica IgG ELISA Test Principle:
The principle of the E. histolytica (Amebiasis) ELISA test is a three-incubation process whereby the first incubation involves the coating of the wells with E. histolytic antigen. Next, the wells must be washed to remove test sample. At this point Enzyme Conjugate is added. During this second incubation, the Enzyme Conjugate will bind to any antibodies present. Then a chromogen TMB is added which develops a blue color in the presence of the enzyme complex and peroxide. The stop solution ends the reaction and turns the blue color to yellow.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the E. histolytica IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.