This Ferritin ELISA test kit is a Quantitative test based on a solid phase enzyme-linked immunosorbent assay that screens for Iron-deficiency anemia.




Materials provided with Ferritin ELISA Test Kit:
1. Antibody-coated ELISA Microplate 96 wells
2. Ferritin EIA test reference standard set, contains 0, 10, 50, 100, 400, &
800 ng/ml(human liver standard) ready for use
3. Ferritin EIA enzyme conjugate reagent
4. TMB Substrate
5. Stop Solution
6. Ferritin ELISA test wash Buffer Concentrate 50X

Materials required but not provided:
1. Precision pipettes & tips
2. EIA kit Microplate Washer
3. EIA kit Microplate Reader





Ferritin ELISA Kit Background Information:
One of the most prevalent disorders in humans is the dietary deficiency of iron and the resulting disease of anemia. Therefore, the assays of iron, total iron binding capacity and other assessments of iron compounds in the body are clinically significant. Iron-storage compounds in the body include hemoglobin, hemosiderin, myoglobulin and the cytochromes. In most tissues, ferritin is a major iron-storage protein. Each molecule of ferritin may contain as many as 4,000 iron atoms. Under normal conditions, this may represent 25% of the total iron found in the body. In addition, ferritin can be found in several isomers. High concentrations of ferritin are found in the cytoplasm of the reticuloendothelial system, the liver, spleen and bone marrow. Methods previously used to measure iron lack adequate sensitivity, and are invasive, causing patient trauma. The ELISA kit measures ferritin in serum, which is useful in determining changes in body iron storage, and is non-invasive with relatively little patient discomfort. Serum ferritin levels can be measured routinely and are particularly useful in the early detection of iron-deficiency anemia in apparently healthy people. Serum ferritin measurements are also clinically significant in the monitoring of the iron status of pregnant women, blood donors, and renal dialysis patients. High ferritin levels may indicate iron overload without apparent liver damage, as may be noted in the early stages of idiopathic hemochromatosis. Ferritin levels in serum have also been used to evaluate clinical conditions not related to iron storage, including inflammation, chronic liver disease, and malignancy.


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Ferritin ELISA Test Principle:
The Ferritin Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti-ferritin antibody for solid phase (microtiter wells) immobilization and another mouse monoclonal anti-ferritin antibody in the antibody-enzyme HRP-conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in the ferritin molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation and washes, a solution of TMB is added and incubated, resulting in the development of a blue color. The color development is stopped with the addition of 2N HCl, and the color is changed to yellow and measured
spectrophotometrically at 450 nm.


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