Folate Elisa kit is a reliable method for the quantitative determination of Folate Concentration in Human Serum by a colorimetric microplate Enzyme Immunoassay.




Materials provided with Folate Elisa kit:
1. Folate Calibrators: Six vials of human serum reference
2. Folate Enzyme Reagent: Folate Analog- HRP conjugate
3. Folate Biotin Reagent
4. Microplate: wells coated with Streptavidin
5. Wash Solution Concentrate
6. Substrate Reagent
7. Stop Solution
8. Releasing Agent
9. Stabilizing Agent
10. Neutralizing Buffer
11. Product Insert

Materials Required, not Provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm and 620nm wavelength




Folate Elisa kit Background Information:
Folate supplementation has escalated over recent years with the knowledge of its many benefits. As one of the B vitamins, folate, or Vitamin B9, is involved in many bodily functions and deficiency can cause disease in not only the elderly, but infants too. Folate deficiency is associated with megaloblastic anemia, neural tube defects, and cardiovascular diseases. Folate plays an important role in brain development and therefore is vital during growth. The most common defects resultant from folate deficiencies are neural tube defects. With a vital role in nucleic acid synthesis, folate has been found to be feneficial as supplementation during pregnancy and other time of rapid tissue growth. Folate also plays a vital role in maintaining proper balance of homocysteine, a contributing factor in occurrences of occlusive vascular diseases and stroke. Individuals with susceptibility to heart disease and several forms of cancer may also benefit from supplementation.




Folate Elisa Test principle:
This Folate Elisa kit is based on the Competitive Enzyme Immunoassay (TYPE 8): The essential reagents required for a Competitive binding assay include specific binding protein, enzyme-antigen conjugate and native antigen. Upon mixing enzyme-antigen conjugate, biotinylated binding protein and a serum containing the native antigen, a competition reaction results between the native antigen and the enzyme-antigen conjugate for a limited number of binding sites. For additional details please refer to the instructions for use.




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