The free beta hCG ELISA kit is a quantitative assay designed for in vitro quantitative measurement of human chorionic gonadotropin free beta-subunit in human serum.



Material Provided with free beta hCG ELISA Kit:
1. Antibody-coated microtiter wells.
2. Reference standards,0,2.5,5,10,25,50 mIU/ml
3. Zero Buffer (Sample diluent)
4. Enzyme Conjugate Reagent
5. TMB Substrate
6. Wash Buffer Concentrate 50X
7. Stop Solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




Free beta hCG ELISA Kit Background Information:
Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone normally produced by placenta during pregnancy. Furthermore, some malignant tumors also produce this hormone, thus elevated levels in non-pregnant patients can lead to diagnosis of cancer. In pregnancy, this hormone is present in blood and urine around seven to thirteen days following implantation of the fertilized ovum. Recent studies showed a significant increase in the level of free Beta-hCG subunit in trisomy 21 cases as compared with controls. Hence, it has been suggested that free Beta-hCG subunit assay in a combination of maternal serum AFP could be effective in a screening protocol for trisomy 21.




Free beta hCG ELISA Test Principle:
The free b-hCG Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti-b-hCG antibody for solid phase (microtiter wells) immobilization and another mouse monoclonal anti-b-hCG antibody in the antibody-enzyme horseradish peroxidase conjugate solution. The serum is added to the b-hCG antibody coated microtiterwells and incubated with the Zero Buffer. If b- hCG is present in the specimen, it will combine with the antibody on the well. For additional details please refer to the instructions for use.


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Notable features of Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Cancer ELISA kits follow a similar method. See the free beta hCG ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .