Giardia Stool Antigen ELISA is an in vitro immunoassay for the qualitative determination of Giardia antigen in fecal specimens.




Material Provided with Giardia ELISA kit:
1.Test Strips Microwells: 96 test wells containing anti-Giardia monoclonal antibodies
2. Enzyme Conjugate: anti-Giardia polyclonal antibodies -HRP
3. Positive Control: diluted Giardia positive antigen formalinized stool supernatant
4. Negative Control: buffer
5. TMB Substrate Solution: TMB
6. Wash Concentrate 20X
7. Dilution Buffer
8. Stop Solution: 5% phosphoric acid

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




Giardia ELISA kit Background Information:
Giardia lamblia is the protozoan parasite responsible for the disease giardiasis. Symptoms of acute giardiasis include diarrhea, nausea, weight loss, malabsorption, abdominal cramps, flatulence and anemia. The disease may manifest itself as an acute, chronic or as an asymptomatic infection. Giardiasis is the most prevalent parasitic disease in the United States and is responsible for an estimated 100 million mild infections and 1 million severe infections each year. The mode of transmission of Giardia is through fecal-oral ingestion of cysts. Epidemics of giardiasis have been documented in day care centers and by drinking contaminated water. Day care centers may be directly or indirectly responsible for 45% of diagnosed Giardia infections in the United States.4 One study found 54% of the children at a day care center were infected. Another important source of Giardia infection is among homosexual men.




Giardia ELISA Test Principle:
During the first incubation, Giardia specific antigen present in the stool specimens are captured by monoclonal antibodies attached to the microwells. The wells are incubated and washed before anti-Giardia polyclonal antibodies conjugated to horseradish peroxidase are added. The enzyme conjugate will sandwich any antigen bound to the wells. After washings to remove unbound enzyme, a chromogen is added which develops a blue color in the presence of the enzyme
complex. The stop solution ends the reaction.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Giardia ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.