The Diagnostic Automation Japanese Encephalitis IgG ELISA test for exposure to Japanese
Encephalitis Virus (JEV) is an ELISA system for the detection of antibodies in human serum to JEVderived recombinant antigen (JERA)




Material Provided with Japanese Encephalitis IgG ELISA kit:
1. Test Strips Microwells: 96 test wells containing JEV Human IgG
2. Sample Dilution Buffer for IgG
3 JE IgG Positive Control: JE IgG Positive Control
4. JE Negative Control: JE Negative Control
5. Enzyme Conjugate-HRP for JE IgG: pre-diluted goat anti-human IgG-HRP
6. 10X Wash Buffer
7. Wash Solution
8. Liquid TMB
9. Stop Solution

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




JE IgG ELISA kit Background Information:
Exposure to JEV causes a disease with a number of symptoms including encephalitis. The JE IgG assay employs a recombinant antigen called JERA, which can be used as a rapid serological marker for JEV infection. The JERA protein is a recombinant antigen, which consists of a stretch of peptides from different parts of the JEV.




JE IgG ELISA Test Principle:
The Diagnostic Automation Inc. Japanese Encephalitis IgG ELISA consists of one enzymatically amplified two-step sandwich-type immunoassay. JE IgG Positive Control (represents reactive or equivocally reactive serum), JE Negative Control (represents non-reactive serum), and unknown serum samples are diluted with Sample Dilution Buffer for IgG then incubated in microtitration wells.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results


Product inserts for most Parasitology ELISA kits follow a similar method. See the Japanese Encephalitis IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.