The Diagnostic Automation Japanese Encephalitis IgM ELISA test for exposure to Japanese
Encephalitis Virus (JEV) is an ELISA system for the detection of antibodies in human serum to JEVderived recombinant antigen (JERA)




Material Provided with Japanese Encephalitis IgG ELISA kit:
1. Test Strips Microwells: 96 test wells containing antibody to Human IgM
2. Sample Dilution Buffer for IgM
3 JE IgM Positive Control: JE IgM Positive Control
4. JE Negative Control: JE Negative Control
5. JE Antigen (JERA) for IgM: pre-diluted JERA solution
6. normal cell antigen (NCA) for JE IgM: pre-diluted NCA solution
7. Enzyme Conjugate-HRP for JE IgG:pre-diluted HRPconjugated flavivirus reactive mAb
8. 10X Wash Buffer
9. En Wash
10. Liquid TMB Substrate
11. Stop Solution

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




JE IgM ELISA kit Background Information:
Exposure to JEV causes a disease with a number of symptoms including encephalitis. The JE IgG assay employs a recombinant antigen called JERA, which can be used as a rapid serological marker for JEV infection. The JERA protein is a recombinant antigen, which consists of a stretch of peptides from different parts of the JEV.




JE IgM ELISA Test Principle:
The Japanese Encephalitis IgM ELISA consists of one enzymatically amplified two-step sandwichtype immunoassay. In this assay, JE Detect Negative Control (Represents non-reactive serum), JE Detect IgM Positive Control (Represents reactive serum), and unknown serum samples are incubated in microtitre wells which have been coated with anti-human IgM antibodies, followed by incubation with both JEV derived recombinant JERA and Normal Cell Antigen (NCA) separately.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Japanese Encephalitis IgM ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.