The Diagnostic Automation Legionella ELISA test kit is an enzyme-linked immunosorbent assay for the qualitative detection of total antibody (IgG/IgM/IgA) to Legionella pneumophila serogroups 1-6 in human sera.




Materials Provided with Legionella IgG/IgM/IgA elisa kit:
1. Plate: wells coated with Formalin-inactivated preparation
of L. pneumophila group 1-6 antigens
2. HRP-Conjugate goat anti-human IgG/IgA/IgM
3. Positive Control: Monkey Serum
4. Calibrator: Monkey Serum
5. Negative Control: Human Serum
6. Sample Diluent
7. TMB
8. Stop Solution: containing 1M H2SO4, 0.7MHCI
9. Wash Buffer Concentrate 10X
10. Package Insert

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




Legionella IgG/IgM/IgA elisa kit Background Information:
L. pneumophila displays a multitude of morphologies including the bacillus, coccobacillus, and elongated fusiform. Although often difficult to perform, the Gram stain will be Gram-negative. The antibody response to L. pneumophila may be both specific and nonspecific, since the patient may have antibodies to similar antigens from other Gram-negative bacteria. Optimum times for specimen collection appear to be within the first week of illness, or as soon as possible after the onset (acute specimen), and at least 3 weeks after the onset (convalescent specimen). By the IFA method, a single result of >1:256 is considered presumptive evidence of legionella infection. Diagnostic titers have been reported to be absent in as many as 25% of patients, but the use of multiple Legionella species as the antigen source and a polyvalent conjugate directed against IgG, IgM, and IgA maximize the accuracy of serological procedures.




Legionella IgG/IgM/IgA ELISA Test Principle:
Legionella IgG/IgA/IgM ELISA test kit is designed for Legionella pneumophila detection of antibodies in human sera. Wells of plastic microwell strips are sensitized by passive absorption with Legionella antigen. The test procedure involves three incubation steps. For additional details please refer to the instructions for use.

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