The DAI Leptin Enzyme Immunoassay Kit is used for the quantitative determination of Leptin in human serum and plasma.




Materials Provided with Leptin Elisa Test Kit:
Microtiter strips: wells coated with anti-Leptin monoclonal antibody
2. Standard (Standard 0-5):
Concentrations: 0, 2, 5, 25, 50 and 100 ng/ml
3. Control: ready to use; 2 levels (low and high)
4. Assay Buffer: ready to use contains 0.3% Proclin as a preservative
5. Antiserum: ready to use polyclonal Leptin antiserum
6. Enzyme Complex: ready to use Anti-rabbit complex conjugated to horseradish Peroxidase
7. Substrate Solution: ready to use TMB
8. Stop Solution: ready to use 0.5M H2SO4
9. Wash Solution: 40X concentrated

Materials required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength
5. Quality Control Materials



Leptin Elisa Test Background Information:
Leptin is from the Greek word Leptos which means thin. This hormone is made by fat cells and regulates the amount of fat stored in the body. Leptin exerts this effect by adjusting both the sensation of hunger and of energy expeditures. Hunger is inhibited when the amount of fat stored reaches a certain level. Leptin is then secreted and circulates through the body, eventually activating leptin receptors in the hypothalamus. Energy expenditure is increased both by the signal to the brain, and directly via leptin receptors on peripheral targets. Although leptin reduces appetite as a circulating signal, obese individuals generally exhibit a higher circulating concentration of leptin than normal weight individuals due to their higher percentage body fats. These people show resistance to leptin, similar to resistance to insulin in type 2 diabetes. Leptin, also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells which have leptin receptors. Many of these additional functions are yet to be defined.




Leptin Elisa Test Principle:
The Diagnostic Automation Leptin ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on a Leptin molecule. An aliquot of patient sample containing endogenous Leptin is incubated in the coated well with a specific rabbit anti Leptin antibody. A sandwich complex is formed.After incubation the unbound material is washed off and an anti rabbit peroxidase conjugate is added for detection of the bound Leptin. Having added the substrate solution, the intensity of color developed is proportional to the concentration of Leptin in the patient sample.



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