The Diagnostic Automation Mumps IgM ELISA kit is intended for the qualitative detection of Mumps IgM antibody in human serum.




Materials Provided with Mumps IgM ELISA Kit:
1. Microassay plate: 96 wells coated with purified Mumps antigen
2. Calibrator: Human serum or defibrinated plasma
3. Positive Control: Human serum or defibrinated plasma
4. Negative Control: Human serum or defibrinated plasma
5. HRP Conjugate: Ready to use, Goat anti-human IgM
6. Serum Diluent PlusI
7. Wash Buffer Type I, 20X
8. Chromogen/Substrate Solution Type I
9. Stop Solution: Ready to use,1N H2SO4 solution

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450 single & 600-650nm dual wavelength




Mumpss IgM ELISA Kit Background Information:
The mumps virus is a member of the paramyxovirus group and the etiological agent of mumps in man. Mumps is a generalized illness usually accompanied by parotid (salivary gland) swelling and mild symptoms. It is also one of the most common causes of aseptic meningitis, encephalitis, and inflammation of the testes (orchitis), pancreas, and ovaries. Current methods for serodiagnosis of mumps infections are in-vitro serum neutralization, hemagglutination-inhibition (HAI), indirect immunofluorescence, and complement fixation (CF) tests. Of these methods, neutralization is reportedly the most specific. However, the neutralization test requires 4-5 days to complete the test. HAI and CF are reportedly less sensitive than the neutralization test. These methods lack specificity, which limits their usefulness in determining immune status. The HAI test also requires pretreatment of test sera to remove nonspecific hemagglutination inhibitors from some sera. Anti-Mumps virus IgM appear 2-3 days after the occurrence of the first clinical symptoms (these remain 2-3 months), followed by the production of Mumps IgG antibodies which persist lifelong following vaccination with live virus there is a seroconversion in 90% of cases, however, the titre is somewhat lower than in normal infections. ELISA can be both specific and sensitive for the detection and measurement of serum proteins. The sensitivity, specificity, and reproducibility of elisa test can be comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination and neutralization.



Mumps IgM ELISA Test Principle:
The Mumps test kit results are read by a microwell reader compared in a parallel manner with calibrator and controls. Diluted patient serum is added to wells, and the Mumps IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.



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