The Diagnostic Automation Mycoplasma Pneumonia IgM ELISA kit is intended for the qualitative determination of IgM antibodies in human serum to Mycoplasma pneumonia antigen. The DAI anti- Mycoplasma IgM assay may be used as an aid in the diagnosis of Mycoplasma pneumoniae infection in the adult population




Materials Provided with Mycoplasma Pneumonia IgM ELISA Kit:
1. Microassay plate:wells coated with inactivated M. pneumonia (strain FH). antigen
2. HRP Conjugate: Ready to use, Goat anti-human IgM
3. Positive Control: Human serum
4. Calibrator: Human serum
5. Negative Control: Human serum
6. Serum Diluent Type I, Ready to use
7. TMB, Ready to use
8. Stop Solution: Ready to use,1N H2SO4 solution
9. Wash Buffer 10X

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450 single & 600-650nm dual wavelength



Mycoplasma Pneumonia IgM ELISA Kit Background Information:
Mycoplasma pneumoniae is among the smallest of free-living organisms. In humans, it is usually found in the throat and lungs and is susceptible to antimicrobial agents that inhibit protein synthesis. The spread of mycoplasmal infections depends on close and prolonged contact between people, as it is transmitted by means of droplet nuclei from the respiratory tract. Mycoplasmal infection may be asymptomatic or may produce upper respiratory tract disease or atypical pneumonia. The pneumonia is difficult to differentiate from viral diseases by clinical means alone Laboratory tests such as isolation, Complement Fixation serology, and ELISA serology are helpful as an aid in the diagnosis.


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Mycoplasma Pneumonia IgM ELISA Test Principle:
When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen- antibody complexes. Excess antibody is removed by washing. This is followed by the addition of goat anti-human IgM globulin conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes. The excess conjugate is removed by washing, followed by the addition of substrate and chromogen. If specific antibody to the antigen is present in the serum, a blue color develops which is proportional to the concentration of antibody in the serum.





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