The Neuron-Specific Enolase (NSE) ELISA provides quantitative measurement of human NSE in serum to aid in the clinical evaluation of patients suspected of having small cell lung cancer, and other related diseases.




Material Provided with NSE ELISA Kit:
1. Microtiter wells coated with Monoclonal anti-NSE antibody
2. Sample diluent
3. Enzyme conjugate reagent
4. Lyophilized NSE reference standards
5. 50 X Wash Buffer Concentrate
6. TMB Substrate
7. Stop Solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




NSE ELISA Kit Background Information:
Neuron-specific enolase (NSE) initially were detected in neurons and neuronendocrine cells. Elevated levels of NSE are commonly found in patients with malignant tumors with neuronendocrine differentiation, especially small cell lung cancer and neuroblastoma. Lung cancer is one of the most spread form of cancer. Patients with small cell lung cancer show various proportions of ag and gg isoenzyme. The determination of NSE should detect and g isoforms with the same sensitivity. The antibodies for this particular assay are specific for the g-subunit without cross reactivity with a or b subunits. NSE Kits are reported to be useful diagnostic marker for lung cancer, neuroblastoma, melanoma, seminoma, and in injury of central nervous system. NSE also can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.




NSE ELISA Test Principle:
The NSE Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one monoclonal anti-gNSE antibody for microtiter wells immobilization and another monoclonal anti-gNSE antibody in the antibody-enzyme horseradish peroxidase conjugate solution. The standards and patients serum are added to the antibody coated microtiter wells. After incubation and washing, the enzymatic reaction develops a color which is proportional to the amount of CEA molecules present in the assay. For additional details please refer to the instructions for use.

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Notable features of Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Cancer ELISA kits follow a similar method. See the NSE ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.


Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .