The Diagnostic Automation Progesterone ELISA Test kit is a Competitive immunoenzymatic colorimetric method for quantitative determination of Progesterone concentration in serum and plasma.





Material Provided with Progesterone ELISA Kit:
1. Microplate: wells coated with goat anti-rabbit IgG antibody
2. Reference Standard Set: 0, 0.5, 3.0, 10, 25, &50 ng/ml of progesterone
3. Rabbit Anti-Progesterone Reagent: rabit anti-progesterone
4. Progesterone Conjugate Concentrate: progesterone HRP-conjugated
5. Progesterone Conjugate Diluent
6. Pregesterone Control 1 & 2: progesterone in human serum
7. TMB Reagent
8. Stop Solution: 1N HCL

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm





Progesterone ELISA Kit Background Information:
Progesterone is a C21 steroid which is synthesized from both tissue and circulating cholesterol. The principle production sites are the adrenals and ovaries and the placenta during pregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as a glucuronide prior to excretion by the kidneys. Progesterone exhibits a wide variety of end organ effects. The primary role of progesterone is exhibited by the reproductive organs. The concentration then increases rapidly following ovulation and remains elevated for 4-6 days and decreases to the initial level 24 hours before the onset of menstruation. In pregnancy, placental progesterone production rises steadily to levels of 10 to 20 times those of the luteal phase peak. Progesterone measurements are thus performed to determine ovulation as well as to characterize luteal phase defects. Monitoring of progesterone therapy and early stage pregnancy evaluations comprise the remaining uses of progesterone assays.





Progesterone ELISA Test Principle:
The Diagnostic Automation progesterone EIA is based on the principle of competitive binding between progesterone in the test specimen and progesterone-HRP conjugate for a constant amount of rabbit anti-progesterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with progesterone standards, controls, patient samples, progesterone-HRPConjugate Reagent and rabbit anti-progesterone reagent at room temperature. After washing, the enzyme substrate are added color development occurs, reaction is stopped and the absorbances are determined. Progesterone concentration in the sample is calculated. For additional details please refer to the instructions for use.







Diagnostic Automation Inc. also provides other Steroid ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .