The Rotavirus Stool Antigen ELISA kit is an in vitro assay for the qualitative determination of rotavirus antigen in feces. It is a double antibody (sandwich) ELISA using a polyclonal anti-rotavirus antibody to capture the antigen from the stool supernatant.




Material Provided with Rotavirus Stool Antigen ELISA kit:
1. Test Strips Microwells: 96 test wells containing anti-Rotavirus antibodies
2. Reagent 1: anti-Rotavirus antibodies with blue dye
3. Reagent 2: antibodies conjugated to HRP with red dye
4. Positive Control: reactive control
5. Negative Control: buffer
6. Chromogen: TMB and peroxide
7. Wash Concentrate (20X)
8. Stop Solution: 5% phosphoric acid

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




Rotavirus Stool ELISA kit Background Information:
Rotavirus is one of the leading causes of gastroenteritis in children throughout the world. Rotavirus infections are most common in infants, but repeated, asymptomatic infections are believed to occur in adults. Rotavirus infection occurs by the fecal-oral route. After an incubation period of 1 - 2 days, the onset of gastroenteritis is sudden. Symptoms can last from 4 - 5 days and range from diarrhea and vomiting, to fever, and occasional abdominal pain. Loss of fluids and electrolytes can lead to severe dehydration, hospitalization, and even death.
Rotavirus infection appears to peak during the winter season, except in countries with tropical or subtropical climates, where the virus is present year around. There have been many efforts to develop rapid and economical methods for detecting rotavirus antigen in stool. Simple to perform assays ELISA kits have become the test of choice in the clinical setting.




Rotavirus Stool ELISA Test Principle:
During the first incubation, Rotavirus antigens present in the stool supernatant are captured by antibodies attached to the wells. The second incubation adds an additional anti-Rotavirus antibody that sandwiches the antigen. The third incubation attaches horseradish peroxidase to the sandwich. After washings to remove unbound enzyme, a chromogen is added which develops a blue color in the presence of the enzyme complex and peroxide. The stop solution ends the reaction and turns the blue color to yellow.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Rotavirus Stool Antigen ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.