Diagnostic Automation offers a Scrub Typhus IgG ELISA Kit which is an immunosorbent assay to test for exposure to Orientia tsutsugamushi (OT; formerly Rickettsia). The Scrub Typhus IgG ELISA Kit is a qualitative, membrane-based immunoassay for the detection of IgG antibodies in human plasma/serum to OT derived recombinant antigen.





Materials Provided with Scrub Typhus IgG ELISA Kit:
1. S Typhus IgG EIA Plate: OT derived recombinant antigens
2. Sample Dilution Buffer for Scrub Typhus
3. Scrub Typhus IgG Positive Control
4. Scrub Typhus Negative Control
5. Ready to Use Enzyme Conjugate-HRP for Scrub Typhus IgG
6. 10X Wash Buffer
7. HRP Wash Solution
8. Liquid TMB Substrate
9. Stop Solution

Materials & Instrumentations required not included:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




S Typhus IgG ELISA Assay Kit Background Information:
Scrub Typhus is an infectious disease that is caused by Orientia tsutsugamushi, formerly known as Rickettsia, a tiny parasite about the size of bacteria that belongs to the family Rickettsiaceae. A bite from the larval trombiculid mite, a parasite of rodents, will transmit the disease. An ulcer of the skin is characteristic of a bite from a trombiculid mite, followed by symptoms including fever, a spotted rash on the torso, and swelling of the lymph glands. Scrub typhus generally occurs after exposure to areas with secondary (scrub) vegetation, from which its name is derived. However, the disease can also be prevalent in sandy, mountainous, and tropical areas. Scrub Typhus is a worldwide illness, but particular to South East Asia and the Western Pacific. It accounts for approximately 20% of fever in some regions in South East Asia, where it is endemic. Illness lasts for a period of 10 to 12 days after the initial bite. With therapy, the fever will break within 36 hours, but if left untreated, complications or death may occur.





S Typhus IgG ELISA Test Principle:
Wells of each plate on the Scrub Typhus IgG ELISA test have been coated with unique recombinant antigen mix. The serum sample is diluted in DAI sample diluent and applied to each well. After incubation and washing, the wells of this Scrub Typhus IgG ELISA Kit are treated with IgG enzyme conjugate (HRP). After a second incubation and washing step, TMB substrat is added. An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450nm. The absorbance measured is directly proportional to the concentration of IgG antibodies to OT present on the S. typhus IgG ELISA kit. For additional details please refer to the instructions for use.



Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Scrub Typhus IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.