Diagnostic Automation Inc. offers a Syphilis (TPA) IgG/IgM ELISA kit, which is an enzyme-linked immunosorbent assay for the qualitative detection of antibodies to Treponema palladium (TP) in serum or plasma. The Syphilis (TPA) IgG/IgM ELISA kit, like many Blood Bank ELISA kits, is for in-vitro diagnostic use only. As in other Blood Bank ELISA Kits, the Syphilis IgG/IgM ELISA Kit is designed as an aid for screening of blood donors, and as an aid in diagnosis of symptoms related to infection with Syphilis. Specificity of the Syphilis IgG/IgM ELISA kit is 99.91%.


Materials provided with Syphilis (TPA) IgG/IgM ELISA test kit:
1. Microwell Plate: well contains recombinant TP antigens
2. Negative Control: non-reactive for TP
3. Positive Control: antibodies to TP diluted
4. HRP-Conjugate Reagent: HRP-conjugated TP antigens
5. Stock Wash Buffer 20x
6. Chromogen Solution A:Urea peroxide solution
7. Chromogen Solution B:TMB solution
8. Stop Solution: 2M sulfuric acid solution
9. Plastic Sealable Bag: 1 unit
10. Cardboard Plastic Cover: 2sheets
11. Package Insert: 1copy

Materials & Instrumentations Required, not provided
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette & tips
3. Dry incubator or water bath
4. Microshaker for dissolving and mixing
5. EIA kit Microplate Washer
6. EIA kit Microplate Reader at single wavelength 450nm or dual wavelength 450 & 630 nm





Syphilis (TPA) IgG/IgM ELISA test Background Information
Syphilis is a disease, usually sexually transmitted, caused by infection with the
spirochete Treponema pallidum (T.pallidum). Infection is systemic from the outset
and the disease is characterized by periods of latency, often in excess of twenty
years. These features, together with the fact that T. pallidum cannot be isolated
in culture mean that serological techniques play a major role in the diagnosis of syphilis and treatment follow-up. Syphilis IgG/IgM Elisa is an enzyme-linked immunosorbent assay for the qualitative detection of antibodies to Treponema palladium (TP) in human serum/plasma.The Syphilis IgG/IgM enzyme immunoassay format allows use of a micorplate reader which eliminates subjective interpretation of results and the test procedure can be automated for high-volume testing.






Product inserts for most Blood Bank ELISA kits follow a similar method. See the Syphilis (TPA) IgG/IgM ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation. Diagnostic Automation Inc. also provides other Blood Bank ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030.