The Diagnostic Automation Thyroglobulin ELISA is intended for the detection and semi-quantitation of antibodies to thyroglobulin in human sera.





Material Provided with Thyroglobulin ELISA Kit:
1. Neo-T4 Calibrators
2. Whole Blood Controls
3. Neo-T4 Elution Buffer
4. Neo-T4 Conjugate Buffer
5. NT4 Enzyme Reagent
6. Microplate: fT4 Antibody Coated wells
7. Wash Solution Concentrate
8. Substrate Solution
9. Stop Solution
10. Product Instructions

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm





Thyroglobulin ELISA Kit Background Information:
Autoimmune thyroid disease is characterized by the presence of circulating autoantibodies directed to thyroid antigens. The majority can be diagnosed by clinical presentation and their antibody profiles to thyroglobulin and thyroid microsomes. Therefore, immunoassays for thyroid antibodies are useful for diagnostic evaluations of thyroid autoimmune disease.Thyroglobulin is a water soluble glycoprotein that is involved in the storage and synthesis of thyroid hormones. Antibodies to thyroglobulin and or microsomal antigen are present in most patients with goitrous thyroiditis (Hashimoto disease), atrophic thyroiditis (myxedema) and about 70-90% of
Graves disease.


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Thyroglobulin ELISA Test Principle:
The Thyroglobulin test is an Enzyme-Linked Immunosorbent Assay to detect IgG, IgA, and IgM antibodies to Thyroglobulin antigens. Purified Thyroglobulin antigens are attached to microplate wells. When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen-antibody complexes. Excess antibody is removed by washing. This is followed by the addition of goat anti-human IgG,A,M conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes. For additional details please refer to the instructions for use.







Diagnostic Automation Inc. also provides other Thyroid ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .