Almond ELISA kit description:

The Diagnostic Automation, Inc. offers Almond ELISA Test Kit for a Quantitative determination of Almond in Food using enzyme linked immunosorbent assay. This Almond ELISA Test represents a highly sensitive detection system which is particularly capable of the quantification of almond residues in cookies, cereals, ice cream and chocolate.




Materials Provided with Almond ELISA Test Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with anti-almond antibodies
2. Almond Standards (0; 0.4; 1; 4; 10 ppm of almond): 5 vials with 1.0 mL each
3. Conjugate (anti-almond-peroxidase): 15 mL
4. Substrate Solution (TMB): 15 mL
5. Stop Solution (0.5 M H2SO4): 15 mL
6. Extraction and sample dilution buffer (Tris), 10x
7. Washing Solution (PBS + Tween 20): 60 mL as 10x
8. Plastic bag to store unused microtiter strips
9. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




Almond ELISA Kit Background Information:
Almond (Prunus dulcis) belongs to the rosaceae. With 25 % the fraction of proteins in almond is high. Some of these proteins are known for being allergenic. In addition to profilin which is known to be cross-reactive to grass pollen, the almond major protein AMP has the greatest relevancy. AMP is known to be heat resistant making it stable to different production processes. For this reason almond represents an important food allergen. For almond allergic persons hidden almond allergens in food are a critical problem. Already very low amounts of almond can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, almond allergic persons must strictly avoid the consumption of almonds or almond containing food. Cross contamination, mostly in consequence of the production process is often noticed. The chocolate production process is a representative example. This explains why in many cases the existence of almond residues in foods cannot be excluded. For this reason sensitive detection systems for almond residues in foodstuffs are required.



Almond ELISA Test Principle:
The Diagnostic Automation, Inc. Almond quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against almond prote-ins is bound on the surface of a microtiter plate.



Diagnostic Automation Inc. offers several popular Food ELISA Test Kits: Chloramphenicol ELISA Kit, Fumonisin ELISA Kit, Streptomycin ELISA Kit, Aflatoxin M1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.