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Hazelnut ELISA kit


Hazelnut ELISA Test

Full name

Food Hazelnut ELISA Test Kit

Category Name Food ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Peroxidase conjugated
Detection Range Qualitative elisa assay- Positive, Negative Controls

Item #:                    5140-8   Quantity:               

Hazelnut ELISA kit

Hazelnut ELISA kit

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Hazelnut ELISA kit description:

Diagnostic AutomationInc offers a Hazelnut ELISA Kit that is an Enzyme Immunoassay for the Quantitative determination of Hazelnut in food. This Hazelnut ELISA Kit is a rapid and sensitive Food Allergen ELISA Kit which is designed to provide a sensitive and convenient method for screening food samples for hazelnut residue.

Materials Provided with Hazelnut ELISA Kit:
1. Microtiter plate:12 strips with 8 breakable wells coated with anti-hazelnut antibodies
2. Hazelnut Standards (0; 1; 4; 10; 40 ppm of hazelnut): 5 vials with 1.0 mL each
3. Conjugate (anti-hazelnut-peroxidase): 15 mL
4. Substrate Solution (TMB): 15 mL
5. Stop Solution (0.5 M H2SO4): 15 mL
6. Extraction and sample dilution buffer (Tris): 2 x
7. Washing Solution (PBS + Tween 20): 60 mL as 10x
8. Plastic bag to store unused microtiter strips
9. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength

Hazelnut Elisa Kit Background Information:
Hazelnut (Corylus avellana) belongs to the birch plants. With 13%, the fraction of proteins in hazelnuts is high. Many of these proteins are known for being allergenic, such as Cor a 9 and Cor a 11. Most of them are very heat resistant making them stable to different production processes. For this reason, hazelnut represents an important food allergen. For hazelnut allergic persons, hidden hazelnut allergens in food are a critical problem. Already very low amounts of hazelnuts can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, hazelnut allergic persons must strictly avoid the consumption of hazelnuts or hazelnut-containing food. Cross contamination, mostly in consequence of the production process is often noticed. The chocolate production process is a representation example. This explains why in many cases the existence of hazelnut residues in foods cannot be excluded. For this reason sensitive detection systems, like this Hazelnut ELISA Kit, for hazelnut residues in foodstuffs are required.

Hazelnut Elisa Test Principle:
This Hazelnut ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. An antibody directed against hazelnut proteins is bound on the surface of a microtiter plate. Hazelnut containing samples or standards are given into the wells of the microtiter plate in the Hazelnut ELISA Kit.

Diagnostic Automation Inc offers several popular Food Allergen ELISA Kits: Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Peanut ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

Due to high risk of cross-contamination all applied instruments like applicator, mortar, glass vials etc. have to be cleaned thoroughly before and after each sample. Hazelnut proteins adhere very strongly to different surfaces. In certain cases they can resist a common dishwasher cleaning. To identify possible cross-contamination caused by previous extractions Diagnostic Automation, Inc. strongly recommends noting the sequence of the extractions. The limit of detection (LOD) of the DAI Hazelnut test is 0.3 ppm. The limit of quantification (LOQ) of the DAI Hazelnut test is 1 ppm.