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Human Prostatic Acid Phosphatase (PAP) ELISA Test Kit

Category Name Cancer ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range 0-60 ng/mL
Sample 50ul serum/plasma
Specificity 98.70%
Sensitivity 0.2 ng/mL
Total Time ~75min
Shelf Life 12 Months from the manufacturing date

Item #:                    4227   Quantity:               


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PAP ELISA kit description:

The prostatic acid phosphatase (PAP) ELISA test kit provides a quantitative measurement of PAP in human serum and plasma.

Material Provided with PAP ELISA Kit:
1. Microtiter wells coated with Anti-PAP antibodies
2. Sample diluent
3. Calibrator Set: 0, 1.5, 5, 15, 30, and 60 ng/mL
4. Control Set: Negative and positive Controls
5. Washing Concentrate 20X
6. Enzyme conjugate: Anti-PAP antibodies HRP-conjugate
7. TMB Chromogenic Substrate
8. Stop Solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm

PAP ELISA Kit Background Information:
In 1938, Gutman and Gutman reported elevated serum acid phosphatase activity in prostatic cancer patients, especially those with bone metastasis. Subsequent studies confirmed that this increased enzyme activity was of prostatic origin; also, the properties of this prostatic enzyme differed from those of acid phosphatase in normal serum. For many years serum acid phosphatase has been measured by spectrophotometric assays based on enzyme activity. These colorimetric methods utilize various substrates, some in conjunction with differential inhibitors of prostatic acid phosphatase. Generally, these assays lack sensitivity or specificity; also, the stability of serum enzymatic activity is time, temperature, and pH dependent. ELISA has been developed to provide a method of high sensitivity and specificity.

PAP ELISA Test Principle:
Diagnostic Automation ELISA PAP is a microwell enzyme linked immunosorbent assay based on the sandwich principle. The wells are coated with anti-PAP antibodies. The samples and Standards are incubated in coated wells. During incubation, if antigen PAP is present, a complex is formed on the well. After incubation and washing, the enzymatic reaction develops a color which is proportional to the amount of CEA molecules present in the assay. For additional details please refer to the instructions for use.

Notable features of Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Cancer ELISA kits follow a similar method. See the PAP ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .