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Penicillin ELISA kit

Name

Penicillin ELISA Test

Full name

Food Penicillin ELISA Test Kit

Category Name Food ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle - Peroxidase conjugated
Sensitivity 3 ng/mL
Total Time 140 min.

Item #:                    5127-8   Quantity:               

Penicillin ELISA kit


Penicillin ELISA kit

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Penicillin ELISA kit description:




Diagnostic Automation Inc. offers a Penicillin Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative determination of Penicillin in Food. This Penicillin Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect penicillin in food.




Materials Provided with Penicillin Food ELISA Kit:
1. Microtiter plate:12 strips with 8 breakable wells coated with penicillin conjugate
2. Penicillin Standards (0; 4; 10; 40; 100; 400 ng/mL): 6 vials with 1.0 mL each
3. Conjugate (anti-mouse-IgG-HRP): 15 mL
4. Anti-Penicillin Antibody (mouse): 6 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample diluent (PBS) 2x
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Two plastic foils to cover the strips during the incubation
10. Plastic bag to store unused microtiter strips
11. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




Penicillin Food ELISA Test Kit Background Information:
Penicillin was accidentally detected by Alexander Fleming in 1929. The drug belongs to the mycotoxins and is generated by the mould Penicillium chrysogenum. Penicillin as an antibiotic is preferentially used in the treatment of gram-positive bacteria, both for humans and animals. Of all illegally administered drugs, antibiotics are most frequently used in productive livestock. Contaminations in food or milk are ingested by humans, and can lead to severe infections by pathogen germs which became resistant against penicillin, or to allergies. The allergic reactions appear with different severity, dependent on dose and individual disposition, and showing symptoms from urticaria to anaphylactic shock. During routine testing of milk samples for antibiotics, in more than 90% of the positive cases, betalactam preparations or penicillins are detected. The method of choice for the determination of penicillin contamination in food has always been a microbiological assay. These procedures allow however no quantitative determination and no identification of the antibiotic drug, which is achieved by a sensitive Penicillin Food ELISA test kit or immunoaffinity columns together with HPLC.




Penicillin Food Test Principle:
The Penicillin Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. A penicillin conjugate is bound on the surface of a microtiter plate.



Diagnostic Automation Inc offers several popular Food Allergen ELISA Kits: Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Peanut ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.




Product Note:

The sensitivity of the Diagnostic Automation, Inc. Penicillin ELISA is 3 ng/mL based on the standard curve. The recovery of spiked samples was determined to 90% for milk and 70% for shrimps. The intra-assay variation of the penicillin test was determined to 3%. Store test kit at 2-8 C. Instrument required but not provided by DAI for this assay are: 50, 100 and 1000 uL micropipets, Microtiter plate shaker, ELISA microplate reader (450 nm), Ultra-turrax or mixer and Centrifuge.